Worthington is competing for the International Award for Best Male for his role in blockbuster Avatar, going up against Simon Baker, Ryan Kwanten and Kodi Smit-McPhee.
Other stars nominated for prizes at the upcoming ceremony include Clive Owen, who is up for the AFI Award For Best Lead Actor for his role in The Boys Are Back, alongside contenders Ben Mendelsohn, James Frecheville and Brendan Cowell.
Abbie Cornish has received a nod for the AFI Award For Best Lead Actress and The Hurt Locker's Guy Pearce is heading the nominations for the Best Supporting Actor trophy.
Movie Animal Kingdom tops the nominations board with a record 18 category listings, Beneath Hill 60 is up for 12 awards and Jane Campion's Bright Star will compete for 11 prizes.
The ceremony is due to take place on 10 and 11 December (10) in Melbourne, Australia.