Fans were given an extra special treat during the performance when Gwyneth Paltrow, who has cameoed on the hit series as substitute school teacher Holly Holliday, made a surprise appearance.
Naya Rivera, who plays Santana Lopez, announced to the audience that she had been named the sexiest Glee club member during the tour, when Gwyneth Paltrow strutted on stage and challenged her to a sing-off.
The Hollywood star then launched into a rendition of Cee-Lo Green's hit track Forget You, which she performed on the show, before the rest of the cast joined in.
And Gwyneth Paltrow used the appearance to treat some young fans by taking them along to the show.
She wrote on Twitter.com following her slot, "Just took four 7 year olds to glee live london. Somebody pass me something vaguely hallucinogenic."